DrugAdopt · Case study
One finding, traced from the report to the code behind it. Read the full report.
One finding
Tolvaptan blocks the V2 receptor in the kidney’s water-handling pathway. We reanalyzed public kidney-expression data from 12 treated and 12 untreated male Pkd1-knockout mice selected for expression profiling.
Aqp2 RNA was lower in treated mouse kidneys. Aqp2 encodes a water channel downstream of V2 signaling. This exploratory result adds a molecular signal to investigate alongside cyst-fluid secretion and the clinical kidney-volume findings.
Mouse expression analysis
Aqp2 encodes aquaporin-2, a water channel, while Avpr2 encodes the receptor itself.
The Aqp2 probe showed a −0.4017 log2 difference between treated and untreated kidneys.
Exploratory comparison of selected whole-kidney samples.
Avpr2 encodes the V2 receptor targeted by tolvaptan.
Aqp2 · probe 17314833
| Measure | Value |
|---|---|
| RNA difference | −0.4017 log2 units |
| 95% confidence interval | −0.5719 to −0.2314 |
| Adjusted P value | 0.000218 |
Tolvaptan minus untreated. P values use Benjamini–Hochberg adjustment.
Fitted RNA contrast · Python
estimate = coefficients @ c
se = np.sqrt(s2_post) * unscaled
t = estimate / se
p_value = 2 * stats.t.sf(np.abs(t), df_total)
margin = stats.t.ppf(0.975, df_total) * se
